BACTOTYPING E. COLI

BACTOTYPING E. COLI

Product description

Product description:

BactoTyping E. coli contains PCR primer sets for the amplification and detection of porcine E. coli virulence-associated genes. The method is based on an intercalating dye using real-time PCR. Amplification curve and gene specific melting curve at 515 nm indicate the amplification of E. coli specific genes. Screening for porcine E. coli adhesion and toxin genes requires picking of several bacterial colonies per plate.

 

Pathogen information:

Most types of diarrhea are caused by strains of enterotoxigenic E. coli (ETEC). The main virulence factors associated with ETEC in diarrhoea are enterotoxins and fimbrial adhesins. The fimbrial types F4 (K88) and F18 are commonly found in pathogenic E. coli isolated from weaned pigs. F18 is most commonly found in Shiga toxin-producing E. coli (STEC) and the Stx2e variant has been associated with oedema disease in pigs.
The fimbrial types F5 (K99), F6 (987P) and F41 are more frequently associated with E. coli causing neonatal diarrhea. Another virulence factor of ETEC is EAST1, which has also been detected in E. coli of different pathogenic strains, such as ETEC, enteropathogenic E. coli (EPEC) and STEC from humans and animals.

 

Principle of real-time PCR:

A specific DNA sequence of the pathogen genome is amplified by a set of two PCR primers that flank the target region and the generated PCR-product is detected by an intercalating nucleic acid dye with subsequent melt curve analysis.