1Step RT qPCR Probe Kit

1Step RT qPCR Probe Kit

Product description

highQu 1Step RT qPCR mastermixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR.

qPCR mastermixes are based on the small molecular inhibitor technology ALLin™ Hot Start Taq Polymerase allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Probe Mixes are available in three versions – without ROX, with low or high ROX concentration.

Applications

• RT-qPCR assays based on specific probes:
• TaqMan^®, Molecular Beacons, Scorpions™ Probes
• Gene expression analysis, quantification of:
• gDNA, cDNA, viral DNA, low copy number genes

Benefits

• Efficient Reverse transcription & sensitive qPCR in one tube
• Both standard or fast cycling, GC rich templates
• Rapid extension, early Ct
• Supplied with PCR Water