BactoReal® Kit Actinobacillus pleuropneumoniae

BactoReal® Kit Actinobacillus pleuropneumoniae

Product description

Kit contents:

• Detection assay for Actinobacillus pleuropneumoniae
• Detection assay for internal positive control (control of amplification)
• DNA reaction mix (contains uracil-N glycosylase, UNG)
• Positive control for A. pleuropneumoniae
• Water

Background: Actinobacillus pleuropneumoniae (APP) causes respiratory disease in swine (pig haemorrhagic pleuropneumonia, actinobacillosis). It is a gram-negative coccobacillus respiratory pathogen. Clinical signs of the acute disease are dyspnea, coughing, anorexia, depression, fever and sometimes vomiting. The disease can progress very rapidly and death can occur within a few hours. Chronic infections are characterized by cough and pleuritis. Many herds are infected with APP without presenting any clinical evidence of the disease. Carrier pigs harbour APP in their nasal cavities and/or tonsils.

Description: BactoReal^® Kit Actinobacillus pleuropneumoniae is based on the amplification and detection of the apxIVA gene of A. pleuropneumoniae using real-time PCR.

PCR-platforms: BactoReal^® Kit Actinobacillus pleuropneumoniae is developed and validated for the ABI PRISM^® 7500 instrument (Life Technologies), LightCycler^® 480 (Roche) and Mx3005P^® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description