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CHÉE & POOL medium including vitamins
Product code : DUC.C0249.0010 DUC.C0249.0010
In the medium defined by Chée and Pool the original Murashige and Skoog concentration of Chlorine, Iodine and Manganese is decreased, resulting in an improved shoot multiplication of Vitis. Substituting calcium nitrate for calcium chloride…
Packaging Unit
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CHÉE & POOL medium including vitamins
Product description
In the medium defined by Chée and Pool the original Murashige and Skoog concentration of Chlorine, Iodine and Manganese is decreased, resulting in an improved shoot multiplication of Vitis. Substituting calcium nitrate for calcium chloride improved the quality of grapevine shoots produced in culture. Shoot multiplication was dramatically improved by ommitting Iodine and lowering the concen-tration of Manganese. This might be the result of the involvement of both ions in auxin metabolism and tranport.
Composition
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 6.20 | 100.27 |
| MnSO4.H20 | 0.85 | 5.00 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 8.60 | 29.91 |
| Macro Elements | mg/l | mM |
|---|---|---|
| Ca(NO3)2 | 492.30 | 2.99 |
| KH2PO4 | 170.00 | 1.25 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 1650.00 | 20.61 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 10.00 | 55.5 |
| Nicotinic Acid | 1.00 | 8.12 |
| Pyridoxine HCl | 1.00 | 5.00 |
| Thiamine HCl | 1.00 | 3.00 |
Total concentration Micro and Macro elements including -vitamins: 4458.49 mg/l
Chée, R., and Pool, R.M., Improved Inorganic Media Constituents for In Vitro Shoot Multiplication of Vitis, Scientia Horticulturae, 32 (1987) 85-95.
CHÉE & POOL medium including vitamins
Product specifications
Packaging Unit
10l
Brand
Duchefa
CHÉE & POOL medium including vitamins
Product documents