CLC / Ipomoea EP medium

CLC / Ipomoea EP medium

Product description

Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM.

Composition

Micro Elements	mg/l	µM
CoCl2.6H2O	0.025
CuSO4.5H2O	0.025
FeNaEDTA	36.70
H3BO3	6.20
KI	0.83
MnSO4.H20	16.90
Na2MoO4.2H2O	0.25
ZnSO4.7H2O	8.60

Macro Elements	mg/l	mM
CaCl2	332.02
KH2PO4	170.00
KNO3	1900.00
MgSO4	180.54
NH4NO3	800.40

Vitamins	mg/l	µM
myo-Inositol	90.10
Nicotinic acid	1.23
Pyridoxine HCl	1.03
Thiamine HCl	1.69

Total concentration Micro and Macro elements (EP) including -vitamins: 3546.54 mg/l

Cheé R. et al., Optimizing Embryogenic Callus and Embryo Growth of a synthetic seed system for Sweetpotato by varying media nutrient concentrations. J. Am. Soc. Hort. Sci. 117, 663 (1992).